OpuE

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  • Description: proline transporter

Gene name opuE
Synonyms yerK
Essential no
Product proline transporter
Function compatible solute transport
Gene expression levels in SubtiExpress: opuE
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 53 kDa, 9.815
Gene length, protein length 1476 bp, 492 aa
Immediate neighbours sapB, gatC
Sequences Protein DNA DNA_with_flanks
Genetic context
OpuE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OpuE expression.png















Categories containing this gene/protein

general stress proteins (controlled by SigB), transporters/ other, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

SigB regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU06660

Phenotypes of a mutant

  • strong growth disadvantage in high-salinity minimal media lacking proline PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: solute:sodium symporter family (SSS family)
  • Paralogous protein(s): PutP

Extended information on the protein

  • Kinetic information:
    • K(M) for proline: 12 +/- 1 µM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Tamara Hoffmann, Carsten von Blohn, Agnieszka Stanek, Susanne Moses, Helena Barzantny, Erhard Bremer
Synthesis, release, and recapture of compatible solute proline by osmotically stressed Bacillus subtilis cells.
Appl Environ Microbiol: 2012, 78(16);5753-62
[PubMed:22685134] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Susanne Moses, Tatjana Sinner, Adrienne Zaprasis, Nadine Stöveken, Tamara Hoffmann, Boris R Belitsky, Abraham L Sonenshein, Erhard Bremer
Proline utilization by Bacillus subtilis: uptake and catabolism.
J Bacteriol: 2012, 194(4);745-58
[PubMed:22139509] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

F Spiegelhalter, E Bremer
Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A- and sigma B-dependent stress-responsive promoters.
Mol Microbiol: 1998, 29(1);285-96
[PubMed:9701821] [WorldCat.org] [DOI] (P p)

C von Blohn, B Kempf, R M Kappes, E Bremer
Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.
Mol Microbiol: 1997, 25(1);175-87
[PubMed:11902719] [WorldCat.org] [DOI] (P p)