OpuE

• Description: proline transporter
  
  

Gene name	opuE
Synonyms	yerK
Essential	no
Product	proline transporter
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuE
Metabolic function and regulation of this protein in SubtiPathways: opuE
MW, pI	53 kDa, 9.815
Gene length, protein length	1476 bp, 492 aa
Immediate neighbours	sapB, gatC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), transporters/ other, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

SigB regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU06660

Phenotypes of a mutant

• strong growth disadvantage in high-salinity minimal media lacking proline PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: solute:sodium symporter family (SSS family)

• Paralogous protein(s): PutP

Extended information on the protein

• Kinetic information:
  • K(M) for proline: 12 +/- 1 µM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O06493

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: opuE PubMed

• Expression browser: opuE PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • induced by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • available in Erhard Bremer's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage