OpuD

• Description: glycine betaine transporter
  
  

Gene name	opuD
Synonyms	ytfQ
Essential	no
Product	glycine betaine transporter
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuD
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	55 kDa, 9.508
Gene length, protein length	1536 bp, 512 aa
Immediate neighbours	ytfP, yteV
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, coping with hyper-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU30070

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54417

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: opuD PubMed

• Sigma factor: SigA PubMed , SigB PubMed

• Regulation:
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A1052 ( opuD::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

R M Kappes, B Kempf, E Bremer
Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD.
J Bacteriol: 1996, 178(17);5071-9
[PubMed:8752321] [WorldCat.org] [DOI] (P p)