Difference between revisions of "OpuBA"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
{{SubtiWiki regulon|[[GbsR regulon]]}},  
 
{{SubtiWiki regulon|[[GbsR regulon]]}},  
{{SubtiWiki regulon|[[OpcR regulon]]}}
+
{{SubtiWiki regulon|[[OpcR regulon]]}},
 
+
{{SubtiWiki regulon|[[RemA regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[OpuBA]]-([[OpuBB]]-[[OpuBD]])-[[OpuBC]] {{PubMed|10092453}}
+
** [[OpuBA]]-([[OpuBB]]-[[OpuBC]])-[[OpuBD]] {{PubMed|10092453}}
  
 
* '''[[Localization]]:''' associated to the membrane (via [[OpuBB]]-[[OpuBD]]) {{PubMed|10092453}}
 
* '''[[Localization]]:''' associated to the membrane (via [[OpuBB]]-[[OpuBD]]) {{PubMed|10092453}}
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** [[GbsR]]: transcription repression {{PubMed|22408163}}
 
** [[GbsR]]: transcription repression {{PubMed|22408163}}
 
** [[OpcR]]: transcription repression {{PubMed|23960087}}
 
** [[OpcR]]: transcription repression {{PubMed|23960087}}
 +
** [[RemA]]: transcription activation {{PubMed|23646920}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>10092453,9925583,10216873, 21296969 23960087 22408163</pubmed>
+
<pubmed>10092453,9925583,10216873, 21296969 23960087 22408163 23646920</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:53, 22 April 2015

Gene name opuBA
Synonyms proV
Essential no
Product choline ABC transporter (ATP-binding protein)
Function compatible solute transport
Gene expression levels in SubtiExpress: opuBA
Interactions involving this protein in SubtInteract: OpuBA
Metabolic function and regulation of this protein in SubtiPathways:
opuBA
MW, pI 42 kDa, 5.654
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours opuBB, yvaV
Sequences Protein DNA DNA_with_flanks
Genetic context
OpuBA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OpuBA expression.png















Categories containing this gene/protein

ABC transporters, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

GbsR regulon, OpcR regulon, RemA regulon

The gene

Basic information

  • Locus tag: BSU33730

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s): OpuCA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 42 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 109 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 215 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 271 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 161 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Chun-Hao Lee, Tien-Yu Wu, Gwo-Chyuan Shaw
Involvement of OpcR, a GbsR-type transcriptional regulator, in negative regulation of two evolutionarily closely related choline uptake genes in Bacillus subtilis.
Microbiology (Reading): 2013, 159(Pt 10);2087-2096
[PubMed:23960087] [WorldCat.org] [DOI] (I p)

Jared T Winkelman, Anna C Bree, Ashley R Bate, Patrick Eichenberger, Richard L Gourse, Daniel B Kearns
RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis.
Mol Microbiol: 2013, 88(5);984-97
[PubMed:23646920] [WorldCat.org] [DOI] (I p)

Gabriele Nau-Wagner, Daniela Opper, Anne Rolbetzki, Jens Boch, Bettina Kempf, Tamara Hoffmann, Erhard Bremer
Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
J Bacteriol: 2012, 194(10);2703-14
[PubMed:22408163] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

R M Kappes, B Kempf, S Kneip, J Boch, J Gade, J Meier-Wagner, E Bremer
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Mol Microbiol: 1999, 32(1);203-16
[PubMed:10216873] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

G Nau-Wagner, J Boch, Le Good JA, E Bremer
High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis.
Appl Environ Microbiol: 1999, 65(2);560-8
[PubMed:9925583] [WorldCat.org] [DOI] (P p)