oppF

168

oligopeptide [wiki|ABC transporter] (ATP-binding protein)

locus: BSU_11470

Molecular weight: 34.59 kDa

pI: 6.95

Protein length: 305 aa

Gene length: 918 bp

function: initiation of sporulation, competence development

product: oligopeptide [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: oppF, spo0KE

List of homologs in different organisms, belongs to COG1123 (Galperin et al., 2021)

Gene

Coordinates: 1,224,533  1,225,450

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The protein

Catalyzed reaction/ biological activity

• required for the uptake of quorum sensing peptides such as [protein|4FA7D6E6316A6FC71C10C692D125833263894020|phrH] [Pubmed|21908671]

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 6-251) (according to UniProt)

Structure

• [PDB|3DHW] (MetN from E. coli, 33% identity) [pubmed|18621668]
• [AF|P24137]

Paralogous protein(s)

• [protein|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF], [protein|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|ykfD]

[wiki|Localization]

• attached to the cell membrane (via [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[protein|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]) [Pubmed|10092453]

Biological materials

Mutant

• GP2097 (D(''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]'')::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• BKE11470 ([gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTCAACTTCTCTC,  downstream forward: _UP4_TGATTCATCAATCCTTCAAG
• BKK11470 ([gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTCAACTTCTCTC,  downstream forward: _UP4_TGATTCATCAATCCTTCAAG

References

• 36815589,27231947,1901616,10092453,9252573,1899858,21908671,12823818,10383984,25966844,25755103,18621668,37755230