Difference between revisions of "OdhB"

From SubtiWiki
Jump to: navigation, search
Line 42: Line 42:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
Line 85: Line 81:
 
* '''Protein family:''' sodium:bile acid symporter family (according to Swiss-Prot)
 
* '''Protein family:''' sodium:bile acid symporter family (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[AcoC]], [[BkdB]], [[PdhC]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 125: Line 121:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=odhB_2107505_2108758_-1 odhB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=odhB_2107505_2108758_-1 odhB] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|1508153}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1508153}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 19:16, 8 June 2013

  • Description: 2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)

Gene name odhB
Synonyms citM
Essential yes PubMed
Product 2-oxoglutarate dehydrogenase complex

(dihydrolipoamide transsuccinylase, E2 subunit)

Function TCA cycle
Gene expression levels in SubtiExpress: odhB
Interactions involving this protein in SubtInteract: OdhB
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 45 kDa, 4.859
Gene length, protein length 1251 bp, 417 aa
Immediate neighbours yocS, odhA
Sequences Protein DNA DNA_with_flanks
Genetic context
OdhB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OdhB expression.png















Categories containing this gene/protein

carbon core metabolism, essential genes, membrane proteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU19360

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine (according to Swiss-Prot)
  • Protein family: sodium:bile acid symporter family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1C4T (from Escherichia coli bl21, 57% identity, 78% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP1145 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1424 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications