Difference between revisions of "OdhA"

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* '''Locus tag:''' BSU19370
 
* '''Locus tag:''' BSU19370
 
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=odhA_2108774_2111608_-1 Expression]
 
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 10:07, 28 January 2012

  • Description: 2-oxoglutarate dehydrogenase (E1 subunit)

Gene name odhA
Synonyms citK
Essential no
Product 2-oxoglutarate dehydrogenase (E1 subunit)
Function TCA cycle
Interactions involving this protein in SubtInteract: OdhA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 105 kDa, 5.871
Gene length, protein length 2823 bp, 941 aa
Immediate neighbours odhB, yojO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
OdhA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU19370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 (according to Swiss-Prot)
  • Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2JGD (from Escherichia coli, 38% identity, 55% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and regulation

  • Regulation:
  • Regulatory mechanism:


  • Additional information:

Biological materials

  • Mutant: GP671 (spc), GP665 (aphA3), GP684 (cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

K F Sheu, J P Blass
The alpha-ketoglutarate dehydrogenase complex.
Ann N Y Acad Sci: 1999, 893;61-78
[PubMed:10672230] [WorldCat.org] [DOI] (P p)

Original publications