Obg

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  • Description: write here

Gene name obg
Synonyms
Essential Yes
Product GTPase activity
Function may be required to stimulate activity of the phosphorelay that activates Spo0A
MW, pI 47 kDa, 4.859
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours spo0B, pheB
Gene sequence (+200bp) Protein sequence
Genetic context
Obg context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Binds and hydrolyzes GTP and readily exchanges GDP for GTP

  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:

[3]

  • Swiss prot entry:
  • KEGG entry:

[4]

  • E.C. number:

Additional information

The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and regulation

  • Operon:

spo0B-obg

  • Sigma factor:

One SigA-dependent promoter upstream of spo0B

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Buglino J, Shen V, Hakiman P & Lima CD (2002) Structural and biochemical analysis of the Obg GTP binding protein Structure 10: 1581-1592. [5]