nusA

168

transcription termination factor of [wiki|RNA polymerase], facilitates the formation of weak terminator hairpins and compensates for distal U-rich tract interruptions

locus: BSU_16600

Molecular weight: 41.57 kDa

pI: 4.58

Protein length: 371 aa

Gene length: 1116 bp

function: transcription

product: transcription termination factor

essential: yes

synonyms: nusA

List of homologs in different organisms, belongs to COG0195 (Galperin et al., 2021)

Gene

Coordinates: 1,732,281  1,733,396

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• stimulates [wiki|transcription] termination at weak intrinsic terminators [pubmed|27571753]

Protein family

• NusA family (single member, according to UniProt)

[wiki|Domains]

• [wiki|S1 domain] (aa 133-199) (according to UniProt)
• [wiki|KH domain] (aa 301-367) (according to UniProt)

Structure

• [PDB|1HH2] (from ''Thermotoga maritima'', 51% identity) [Pubmed|11430821]
• [AF|P32727]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s lab

References

Reviews

• 26132790,36154805

Original Publications

• 36815589,11948165,8491709,19680289,12161562,10027981,15978071,20817769,11430821,28516784,27571753,33243850,33835023,35258320,36192538