NupN

• Description: lipoprotein, part of guanosine transporter
  
  

Gene name	nupN
Synonyms	yufN
Essential	no
Product	lipoprotein, part of guanosine transporter
Function	uptake of guanosine
Gene expression levels in SubtiExpress: nupN
MW, pI	37 kDa, 4.82
Gene length, protein length	1050 bp, 350 aa
Immediate neighbours	malR, nupO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU31540

Phenotypes of a mutant

• a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

Database entries

• BsubCyc: BSU31540

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: BMP lipoprotein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• BsubCyc: BSU31540

• Structure:

• UniProt: O05252

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nupN-nupO-nupP-nupQ PubMed

• Expression browser: nupN PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Abraham L Sonenshein
CodY-mediated regulation of guanosine uptake in Bacillus subtilis.
J Bacteriol: 2011, 193(22);6276-87
[PubMed:21926227] [WorldCat.org] [DOI] (I p)

Boris R Belitsky, Abraham L Sonenshein
Roadblock repression of transcription by Bacillus subtilis CodY.
J Mol Biol: 2011, 411(4);729-43
[PubMed:21699902] [WorldCat.org] [DOI] (I p)

Shaun R Brinsmade, Roelco J Kleijn, Uwe Sauer, Abraham L Sonenshein
Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools.
J Bacteriol: 2010, 192(24);6357-68
[PubMed:20935095] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)