NupG

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  • Description: purine nucleoside transporter

Gene name nupG
Synonyms yxjA
Essential no
Product purine nucleoside transporter
Function purine uptake
MW, pI 43 kDa, 7.757
Gene length, protein length 1191 bp, 397 aa
Immediate neighbours yxjB, yxiT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YxjA context.gif
This image was kindly provided by SubtiList





Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box

The gene

Basic information

  • Locus tag: BSU39020

Phenotypes of a mutant

  • a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: SLC28A transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: nupG
  • Regulation:
    • expression activated by glucose (4.3 fold) PubMed
    • repressed by hypoxanthine and guanine, PurR-independent, G-boxPubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)