nupG

168

purine nucleoside transporter

locus: BSU_39020

Molecular weight: 43.56 kDa

pI: 7.76

Protein length: 397 aa

Gene length: 1194 bp

function: purine uptake

product: purine nucleoside transporter

essential: no

synonyms: nupG, yxjA

List of homologs in different organisms, belongs to COG1972 (Galperin et al., 2021)

Gene

Coordinates: 4,005,752 → 4,006,945

Phenotypes of a mutant

• a ''[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]

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The protein

Protein family

• concentrative nucleoside transporter (CNT) (TC 2.A.41) family (with [protein|4F718681A7AD0C86B2551248570F493AC0B2E7E9|nupC] and [protein|7D4CF2FF4108D3AE170C1EE3C7D494EAD46699C6|yutK], according to UniProt)

Structure

• [PDB|3TIJ] (from Vibrio cholerae, 25% identity) [pubmed|22407322]
• [AF|P42312]

[wiki|Localization]

• cell membrane [Pubmed|18763711]

Biological materials

Mutant

• MGNA-B725 (yxjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1724 NBRP B. subtilis, Japan]
• CS219 (''[gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE39020 (Δ[gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCAACTCCTATGA,  downstream forward: _UP4_TAGAAACATTGAGCCATATC
• BKK39020 (Δ[gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCAACTCCTATGA,  downstream forward: _UP4_TAGAAACATTGAGCCATATC

References

• 12850135,18763711,12923093,21926227,27197833,22407322,29794222,35348734,35622174