Nth

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  • Description: endonuclease III, removes abasic sites during base excision repair

Gene name nth
Synonyms jooB, ypoB
Essential no
Product endonuclease III
Function DNA repair
MW, pI 24 kDa, 9.277
Gene length, protein length 657 bp, 219 aa
Immediate neighbours ypoC, dnaD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Nth context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22340

Phenotypes of a mutant

  • the mutant is sensititive to exposure to H2O2 PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1P59 (complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • constitutively expressed PubMed
    • expression is transiently increased after treatment with H2O2 PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christopher Collier, Cristina Machón, Geoff S Briggs, Wiep Klaas Smits, Panos Soultanas
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Nucleic Acids Res: 2012, 40(2);739-50
[PubMed:21954439] [WorldCat.org] [DOI] (I p)

J Christopher Fromme, Gregory L Verdine
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J: 2003, 22(13);3461-71
[PubMed:12840008] [WorldCat.org] [DOI] (P p)