Nth

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  • Description: endonuclease III, removes abasic sites during base excision repair

Gene name nth
Synonyms jooB, ypoB
Essential no
Product endonuclease III
Function DNA repair
Gene expression levels in SubtiExpress: nth
MW, pI 24 kDa, 9.277
Gene length, protein length 657 bp, 219 aa
Immediate neighbours ypoC, dnaD
Sequences Protein DNA DNA_with_flanks
Genetic context
Nth context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Nth expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22340

Phenotypes of a mutant

  • the mutant is sensititive to exposure to H2O2 PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1P59 (complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • constitutively expressed PubMed
    • expression is transiently increased after treatment with H2O2 PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Christopher Collier, Cristina Machón, Geoff S Briggs, Wiep Klaas Smits, Panos Soultanas
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Nucleic Acids Res: 2012, 40(2);739-50
[PubMed:21954439] [WorldCat.org] [DOI] (I p)

J Christopher Fromme, Gregory L Verdine
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J: 2003, 22(13);3461-71
[PubMed:12840008] [WorldCat.org] [DOI] (P p)