ntdA

168

pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase

locus: BSU_10550

Molecular weight: 49.98 kDa

pI: 5.97

Protein length: 441 aa

Gene length: 1326 bp

function: synthesis of the antibiotic kanosamine

product: pyridoxal phosphate-dependent

essential: no

synonyms: ntdA, yhjL

List of homologs in different organisms, belongs to COG0399 (Galperin et al., 2021)

Gene

Coordinates: 1,128,286  1,129,611

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The protein

Catalyzed reaction/ biological activity

• 3-oxo-d-glucose-6-phosphate + glutamate --> kanosamine-6-phosphate [Pubmed|23586652]

Protein family

• DegT/DnrJ/EryC1 family (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN] and [protein|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC], according to UniProt)

[wiki|Cofactors]

• pyridoxalphosphate [Pubmed|23586652]

Structure

• [PDB|4K2I] [Pubmed|24097983]
• [AF|O07566]

Biological materials

Mutant

• MGNA-A725 (yhjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/725 NBRP B. subtilis, Japan]
• BKE10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG,  downstream forward: _UP4_TTTCATGTTATTAAGCAAGA
• BKK10550 ([gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACAGTACCTCCAATG,  downstream forward: _UP4_TTTCATGTTATTAAGCAAGA

References

Reviews

• 21512256

Original Publications

• 19087206,19342798,17056753,14612444,23586652,24097983,32786294,33619155,34096710