Difference between revisions of "NsrR"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[transcription factors and their control]]}},
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{{SubtiWiki category|[[resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]]}}
 
=The protein=
 
=The protein=
  

Revision as of 18:28, 30 November 2010

  • Description: nitric oxide-responsive regulator

Gene name nsrR
Synonyms yhdE
Essential no
Product transcriptional repressor
Function repression of ResD-ResE-dependent genes in the absence of nitric oxide (NO)
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 16 kDa, 7.75
Gene length, protein length 438 bp, 146 aa
Immediate neighbours lytF, ygxB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhdE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU09380

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

transcription factors and their control, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): [4Fe-4S] cluster, required for DNA-binding PubMed
  • Effectors of protein activity:
    • nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets PubMed
    • [4Fe-4S]-NsrR binds around the -35 element of the nasD promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sushma Kommineni, Erik Yukl, Takahiro Hayashi, Jacob Delepine, Hao Geng, Pierre Moënne-Loccoz, Michiko M Nakano
Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter.
Mol Microbiol: 2010, 78(5);1280-93
[PubMed:21091510] [WorldCat.org] [DOI] (I p)

Annika Rogstam, Jonas T Larsson, Peter Kjelgaard, Claes von Wachenfeldt
Mechanisms of adaptation to nitrosative stress in Bacillus subtilis.
J Bacteriol: 2007, 189(8);3063-71
[PubMed:17293416] [WorldCat.org] [DOI] (P p)

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed