nrnA

168

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase, degradation of pGpG

locus: BSU_29250

Molecular weight: 34.93 kDa

pI: 4.67

Protein length: 313 aa

Gene length: 942 bp

function: processing of short RNA substrates

product: oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase

essential: no

ec: 3.1.3.7

synonyms: nrnA, ytqI

List of homologs in different organisms, belongs to COG0618 (Galperin et al., 2021)

Gene

Coordinates: 2,995,908  2,996,849

Phenotypes of a mutant

• The mutant requires cysteine [Pubmed|17586819]
• strongly reduced [wiki|genetic competence], competence can be restored by overexpression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]-[gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS] [pubmed|33897624]
• inactivation of [gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA] reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activity, and production of small forespores [Pubmed|26735940]

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The protein

Catalyzed reaction/ biological activity

• oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
• hydrolyzes oligo(deoxy)ribonucleotides in a 5' 3' direction [Pubmed|28894100,21087930]
• hydrolyzes nano-RNAs in a 3' 5' direction [Pubmed|28894100]
• degradation of pGpG derived from c-di-GMP [pubmed|30249708]
• adenosine 3',5'-bisphosphate + H₂O --> AMP + phosphate (according to UniProt)

Protein family

• NrnA oligoribonuclease family (single member, according to UniProt)

[wiki|Domains]

• [wiki|DHH-DHHA1 domain] [pubmed|28894100]

[wiki|Cofactors]

• Mn²⁺ [pubmed|28894100]

Structure

• [PDB|5J21] [pubmed|28894100]
• [AF|O34600]

[wiki|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]

  description: [Pubmed|9387221]

Biological materials

Mutant

• MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
• GP2155 (Δ[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
• BKE29250 ([gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG,  downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
• BKK29250 ([gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG,  downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT

References

Reviews

• 29458847,31464530,36026528

Original Publications

• 17586819,9387221,16479537,19429620,19553197,21087930,22102036,26691161,21768284,24013631,26735940,27834680,28894100,30249708,33897624,36478094