NrgA
- Description: ammonium transporter, required at low ammonium concentration
| Gene name | nrgA |
| Synonyms | amtB |
| Essential | no |
| Product | ammonium transporter |
| Function | ammonium uptake |
| Interactions involving this protein in SubtInteract: NrgA | |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 42 kDa, 5.727 |
| Gene length, protein length | 1212 bp, 404 aa |
| Immediate neighbours | ywoB, nrgB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
transporters/ other, biosynthesis/ acquisition of amino acids, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36510
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot), membrane
Database entries
- UniProt: Q07429
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
Reviews
Arnaud Javelle, Domenico Lupo, Xiao-Dan Li, Mike Merrick, Mohamed Chami, Pierre Ripoche, Fritz K Winkler
Structural and mechanistic aspects of Amt/Rh proteins.
J Struct Biol: 2007, 158(3);472-81
[PubMed:17368911]
[WorldCat.org]
[DOI]
(P p)
G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624]
[WorldCat.org]
[DOI]
(P p)
Original Publications
Matthew J Conroy, Anne Durand, Domenico Lupo, Xiao-Dan Li, Per A Bullough, Fritz K Winkler, Mike Merrick
The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.
Proc Natl Acad Sci U S A: 2007, 104(4);1213-8
[PubMed:17220269]
[WorldCat.org]
[DOI]
(P p)
Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076]
[WorldCat.org]
[DOI]
(P p)
Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241]
[WorldCat.org]
[DOI]
(P p)
Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818]
[WorldCat.org]
[DOI]
(P p)
L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114]
[WorldCat.org]
[DOI]
(P p)
L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685]
[WorldCat.org]
[DOI]
(P p)
M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935]
[WorldCat.org]
[DOI]
(P p)
