nrdF

168

ribonucleoside-diphosphate reductase (beta subunit)

locus: BSU_17390

Molecular weight: 38.22 kDa

pI: 4.66

Protein length: 329 aa

Gene length: 990 bp

function: synthesis of deoxyribonucleoside triphosphates

product: ribonucleoside-diphosphate reductase (beta subunit)

essential: yes

ec: 1.17.4.1

synonyms: nrdF

List of homologs in different organisms, belongs to COG0208 (Galperin et al., 2021)

Gene

Coordinates: 1,871,089 → 1,872,078

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H₂O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)
• required for stationary-phase mutagenesis [Pubmed|27920297]

Protein family

• ribonucleoside diphosphate reductase small chain family (with [protein|08B733574DC7B8D044033EBB94124D470A0FBE19|yosP], according to UniProt)

[wiki|Cofactors]

• dimanganic-tyrosyl radical (Mn^III₂-Y•) cofactor [Pubmed|24401092]

Structure

• [PDB|4DR0] [Pubmed|22443445]
• [PDB|6MW3] (inhibitory filament formed with [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE]) [Pubmed|31201319]
• [AF|P50621]

Effectors of protein activity

• dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of [protein|3B68CE8E9A35997B45E437A316996D585E43B76B|nrdE] [Pubmed|31201319,29712847,24401092]

Paralogous protein(s)

• [protein|08B733574DC7B8D044033EBB94124D470A0FBE19|yosP]

Biological materials

Expression vectors

• pGP2894: expression of Strep-''nrdF'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]

References

Reviews

• 17229208,32569524

Original Publications

• 16885274,8969495,24401092,23402532,22443445,21561096,15949864,27920297,28624879,31201319,34031038,35652913