Difference between revisions of "NrdF"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
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{{SubtiWiki category|[[essential genes]]}}
 
=The protein=
 
=The protein=
  

Revision as of 18:27, 30 November 2010

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdF
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 38 kDa, 4.66
Gene length, protein length 987 bp, 329 aa
Immediate neighbours nrdE, ymaB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NrdF context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU17390

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  • Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
  • Paralogous protein(s): YosP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

Reviews

Original Publications

Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274] [WorldCat.org] [DOI] (P p)

C Scotti, A Valbuzzi, M Perego, A Galizzi, A M Albertini
The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae.
Microbiology (Reading): 1996, 142 ( Pt 11);2995-3004
[PubMed:8969495] [WorldCat.org] [DOI] (P p)