Difference between revisions of "NrdF"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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* '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' {{PubMed|16885274}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16885274}}
  
* '''Regulation:''' distinct promoters for aerobic and anaerobic expression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed], anaerobic expression requires [[ResD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
+
* '''Regulation:'''  
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
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** expressed under anaerobic conditions ([[ResD]]) {{PubMed|16885274}}
  
* '''Regulatory mechanism:''' [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
+
* '''Regulatory mechanism:'''  
** [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
+
** [[ResD]]: transcription activation {{PubMed|16885274}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 20:18, 16 October 2009

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdF
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 38 kDa, 4.66
Gene length, protein length 987 bp, 329 aa
Immediate neighbours nrdE, ymaB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NrdF context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU17390

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  • Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
  • Paralogous protein(s): YosP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274] [WorldCat.org] [DOI] (P p)

C Scotti, A Valbuzzi, M Perego, A Galizzi, A M Albertini
The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae.
Microbiology (Reading): 1996, 142 ( Pt 11);2995-3004
[PubMed:8969495] [WorldCat.org] [DOI] (P p)