Difference between revisions of "Nos"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[nos]]-[[yflK]]'' (according to [http://dbtbs.hgc.jp/COG/prom/yflMK.html DBTBS])
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:''' Note that the gene ''[[yflL]]'' is located between ''[[nos]]'' and ''[[yflK]]'', but on the opposite strand.
  
 
=Biological materials =
 
=Biological materials =

Revision as of 18:03, 31 August 2009

  • Description: nitric-oxide synthase

Gene name nos
Synonyms yflM
Essential no
Product nitric-oxide synthase
Function production of nitric oxide
MW, pI 38 kDa, 5.528
Gene length, protein length 1008 bp, 336 aa
Immediate neighbours yflN, yflL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YflM context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU07630

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Bacterial NOS oxygenase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2AMO (loose dimer), 1M7V (bound to tetrahydrofolate and arginine)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: Note that the gene yflL is located between nos and yflK, but on the opposite strand.

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kartikeya Pant, Alexandrine M Bilwes, Subrata Adak, Dennis J Stuehr, Brian R Crane
Structure of a nitric oxide synthase heme protein from Bacillus subtilis.
Biochemistry: 2002, 41(37);11071-9
[PubMed:12220171] [WorldCat.org] [DOI] (P p)

Subrata Adak, Kulwant S Aulak, Dennis J Stuehr
Direct evidence for nitric oxide production by a nitric-oxide synthase-like protein from Bacillus subtilis.
J Biol Chem: 2002, 277(18);16167-71
[PubMed:11856757] [WorldCat.org] [DOI] (P p)