NifZ

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  • Description: cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA

Gene name nifZ
Synonyms iscSB
Essential no
Product cysteine desulfurase
Function biosynthesis of 4-thiouridine in tRNA
Gene expression levels in SubtiExpress: nifZ
Interactions involving this protein in SubtInteract: NifZ
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 41 kDa, 6.34
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours thiI, braB
Gene sequence (+200bp) Protein sequence
Genetic context
NifZ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NifZ expression.png













Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29590

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: removes sulfuryl group from cysteine and transfers it to ThiI PubMed
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1EG5 (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787] [WorldCat.org] [DOI] (I p)

J T Kaiser, T Clausen, G P Bourenkow, H D Bartunik, S Steinbacher, R Huber
Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.
J Mol Biol: 2000, 297(2);451-64
[PubMed:10715213] [WorldCat.org] [DOI] (P p)