Difference between revisions of "NifS"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU27880
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38033 P38033]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38033 P38033]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27880 BSU27880]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27880]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.8.1.7 2.8.1.7]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.8.1.7 2.8.1.7]

Revision as of 09:46, 3 June 2009

  • Description: cysteine desulfurase, required for NAD biosynthesis

Gene name nifS
Synonyms iscS
Essential no
Product cysteine desulfurase
Function NAD biosynthesis
MW, pI 43 kDa, 6.425
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours nadB, nadR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NifS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)
  • Protein family: NifS/iscS subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed