Difference between revisions of "NfrA"

From SubtiWiki
Jump to: navigation, search
Line 61: Line 61:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU38110&redirect=T BSU38110]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nfrA-ywcH.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nfrA-ywcH.html]
Line 98: Line 99:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU38110&redirect=T BSU38110]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3N2S 3N2S] {{PubMed|20727352}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3N2S 3N2S] {{PubMed|20727352}}

Revision as of 15:07, 2 April 2014

  • Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein

Gene name nfrA
Synonyms ywcG, ipa-43d
Essential no
Product FMN-containing NADPH-linked nitro/flavin reductase
Function unknown
Gene expression levels in SubtiExpress: nfrA
Metabolic function and regulation of this protein in SubtiPathways:
nfrA
MW, pI 28 kDa, 5.732
Gene length, protein length 747 bp, 249 aa
Immediate neighbours ywcH, rodA
Sequences Protein DNA DNA_with_flanks
Genetic context
NfrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NfrA expression.png




























Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigD regulon, Spo0A regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU38110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed
  • Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sylvie Cortial, Philippe Chaignon, Bogdan I Iorga, Stéphane Aymerich, Gilles Truan, Virginie Gueguen-Chaignon, Philippe Meyer, Solange Moréra, Jamal Ouazzani
NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
FEBS Lett: 2010, 584(18);3916-22
[PubMed:20727352] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Haike Antelmann, Christine Eymann, Dirk Albrecht, Jörg Bernhardt, Michael Hecker
Proteome signatures for stress and starvation in Bacillus subtilis as revealed by a 2-D gel image color coding approach.
Proteomics: 2006, 6(16);4565-85
[PubMed:16847875] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
Transcription of the nfrA-ywcH operon from Bacillus subtilis is specifically induced in response to heat.
J Bacteriol: 2000, 182(16);4384-93
[PubMed:10913069] [WorldCat.org] [DOI] (P p)

S Zenno, T Kobori, M Tanokura, K Saigo
Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase.
Biosci Biotechnol Biochem: 1998, 62(10);1978-87
[PubMed:9836433] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
The sigmaD-dependent transcription of the ywcG gene from Bacillus subtilis is dependent on an excess of glucose and glutamate.
Mol Microbiol: 1998, 27(5);889-98
[PubMed:9535080] [WorldCat.org] [DOI] (P p)