Difference between revisions of "NdoA"

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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:''' the activity is inhibited by the interaction with [[NdoAI]] {{PubMed|17416361}}
 
* '''Effectors of protein activity:''' the activity is inhibited by the interaction with [[NdoAI]] {{PubMed|17416361}}
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=References=
 
=References=
  
<pubmed>15882409,17416361, 20525796 21763692 21897862 23378533 14517982 24120662</pubmed>
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<pubmed>15882409,17416361, 20525796 21763692 21897862 23378533 14517982 24120662 24489751 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:35, 6 February 2014

  • Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase

Gene name ndoA
Synonyms ydcE, mazF
Essential no
Product mRNA interferase
Function unknown
Gene expression levels in SubtiExpress: ndoA
Interactions involving this protein in SubtInteract: NdoA
MW, pI 12 kDa, 4.892
Gene length, protein length 348 bp, 116 aa
Immediate neighbours ndoAI, rsbR
Sequences Protein DNA DNA_with_flanks
Genetic context
YdcE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NdoA expression.png















Categories containing this gene/protein

Rnases, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU04660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed
  • Protein family: pemK family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: the activity is inhibited by the interaction with NdoAI PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mariangela Tabone, Virginia S Lioy, Silvia Ayora, Cristina Machón, Juan C Alonso
Role of toxin ζ and starvation responses in the sensitivity to antimicrobials.
PLoS One: 2014, 9(1);e86615
[PubMed:24489751] [WorldCat.org] [DOI] (I e)

Dhirendra K Simanshu, Yoshihiro Yamaguchi, Jung-Ho Park, Masayori Inouye, Dinshaw J Patel
Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis.
Mol Cell: 2013, 52(3);447-58
[PubMed:24120662] [WorldCat.org] [DOI] (I p)

Yojiro Ishida, Jung-Ho Park, Lili Mao, Yoshihiro Yamaguchi, Masayori Inouye
Replacement of all arginine residues with canavanine in MazF-bs mRNA interferase changes its specificity.
J Biol Chem: 2013, 288(11);7564-7571
[PubMed:23378533] [WorldCat.org] [DOI] (I p)

Xiangli Wu, Xiuhong Wang, Karl Drlica, Xilin Zhao
A toxin-antitoxin module in Bacillus subtilis can both mitigate and amplify effects of lethal stress.
PLoS One: 2011, 6(8);e23909
[PubMed:21897862] [WorldCat.org] [DOI] (I p)

Jung-Ho Park, Yoshihiro Yamaguchi, Masayori Inouye
Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.
FEBS Lett: 2011, 585(15);2526-32
[PubMed:21763692] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361] [WorldCat.org] [DOI] (P p)

Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409] [WorldCat.org] [DOI] (P p)

Arhonda Gogos, Haiyan Mu, Fabiana Bahna, Carlos A Gomez, Lawrence Shapiro
Crystal structure of YdcE protein from Bacillus subtilis.
Proteins: 2003, 53(2);320-2
[PubMed:14517982] [WorldCat.org] [DOI] (I p)