Difference between revisions of "NdoA"
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | ** [[NdoAI]]-[[NdoA]] {{PubMed|17416361}} | + | ** [[NdoA]](2)-[[NdoAI]](2)-[[NdoA]](2) {{PubMed|24120662,17416361}} |
| + | ** the active protein forms dimers {{PubMed|24120662}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 90: | Line 91: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1NE8 1NE8] {{PubMed|14517982}} | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=1NE8 1NE8] {{PubMed|14517982}} | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=4MDX 4MDX] (NdoA-RNA) {{PubMed|24120662}} | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=4ME7 4ME7] ([[NdoA]]-[[NdoAI]] complex) {{PubMed|24120662}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P96622 P96622] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P96622 P96622] | ||
| Line 135: | Line 139: | ||
=References= | =References= | ||
| − | <pubmed>15882409,17416361, 20525796 21763692 21897862 23378533 14517982 </pubmed> | + | <pubmed>15882409,17416361, 20525796 21763692 21897862 23378533 14517982 24120662</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:22, 21 October 2013
- Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
| Gene name | ndoA |
| Synonyms | ydcE, mazF |
| Essential | no |
| Product | mRNA interferase |
| Function | unknown |
| Gene expression levels in SubtiExpress: ndoA | |
| Interactions involving this protein in SubtInteract: NdoA | |
| MW, pI | 12 kDa, 4.892 |
| Gene length, protein length | 348 bp, 116 aa |
| Immediate neighbours | ndoAI, rsbR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
Rnases, toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04660
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed
- Protein family: pemK family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
Database entries
- UniProt: P96622
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for ndoA PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Dhirendra K Simanshu, Yoshihiro Yamaguchi, Jung-Ho Park, Masayori Inouye, Dinshaw J Patel
Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis.
Mol Cell: 2013, 52(3);447-58
[PubMed:24120662]
[WorldCat.org]
[DOI]
(I p)
Yojiro Ishida, Jung-Ho Park, Lili Mao, Yoshihiro Yamaguchi, Masayori Inouye
Replacement of all arginine residues with canavanine in MazF-bs mRNA interferase changes its specificity.
J Biol Chem: 2013, 288(11);7564-7571
[PubMed:23378533]
[WorldCat.org]
[DOI]
(I p)
Xiangli Wu, Xiuhong Wang, Karl Drlica, Xilin Zhao
A toxin-antitoxin module in Bacillus subtilis can both mitigate and amplify effects of lethal stress.
PLoS One: 2011, 6(8);e23909
[PubMed:21897862]
[WorldCat.org]
[DOI]
(I p)
Jung-Ho Park, Yoshihiro Yamaguchi, Masayori Inouye
Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.
FEBS Lett: 2011, 585(15);2526-32
[PubMed:21763692]
[WorldCat.org]
[DOI]
(I p)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361]
[WorldCat.org]
[DOI]
(P p)
Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409]
[WorldCat.org]
[DOI]
(P p)
Arhonda Gogos, Haiyan Mu, Fabiana Bahna, Carlos A Gomez, Lawrence Shapiro
Crystal structure of YdcE protein from Bacillus subtilis.
Proteins: 2003, 53(2);320-2
[PubMed:14517982]
[WorldCat.org]
[DOI]
(I p)
