Difference between revisions of "Ndk"

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(Database entries)
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1WKJ 1WKJ] (from ''Thermus thermophilus'', 59% identity, 82% similarity)
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P31103 P31103]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P31103 P31103]

Revision as of 15:46, 19 February 2010

  • Description: nucleoside diphosphate kinase

Gene name ndk
Synonyms
Essential no
Product nucleoside diphosphate kinase
Function maintainance of an equilibrium between the
concentrations of different nucleoside triphosphates
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Purine salvage
MW, pI 16 kDa, 5.568
Gene length, protein length 447 bp, 149 aa
Immediate neighbours cheR, hepT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ndk context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU22730

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate (according to Swiss-Prot)
  • Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Thr-92 AND Ser-123 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1WKJ (from Thermus thermophilus, 59% identity, 82% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)