ndh

168

NADH dehydrogenase (Menaquinone 7 & no proton)

locus: BSU_12290

Molecular weight: 41.79 kDa

pI: 6.29

Protein length: 392 aa

Gene length: 1179 bp

function: respiration

product: NADH dehydrogenase (Menaquinone 7 & no proton)

essential: no

synonyms: ndh, yjlD

List of homologs in different organisms, belongs to COG1252 (Galperin et al., 2021)

Gene

Coordinates: 1,299,074 1,300,252

Phenotypes of a mutant

• inactivation of ''ndh'' facilitates growth without a cell wall (due to reduction of oxidative stress) [Pubmed|26051891]
• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

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The protein

Protein family

• NADH dehydrogenase family (with [protein|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|yutJ] and [protein|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|yumB], according to UniProt)

[wiki|Cofactors]

• FAD [pubmed|31133996]

Structure

• [PDB|4NWZ] (from ''C. thermarum'', 42% identity) [Pubmed|24444429]
• [AF|P80861]

Paralogous protein(s)

• [protein|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|yumB]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• MGNA-A358 (yjlD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/358 NBRP B. subtilis, Japan]
• BKE12290 ([gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG
• BKK12290 ([gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG

References

• 36815589,17015645,20933603,11948165,18763711,15378759,26051891,24444429,28439033,10913079,28189581,31133996