Difference between revisions of "NasC"

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Revision as of 12:30, 16 May 2013

  • Description: nitrate reductase (catalytic subunit)

Gene name nasC
Synonyms narB, nasBB
Essential no
Product nitrate reductase (catalytic subunit)
Function utilization of nitrate
Gene expression levels in SubtiExpress: nasC
Interactions involving this protein in SubtInteract: NasC
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 78 kDa, 6.728
Gene length, protein length 2130 bp, 710 aa
Immediate neighbours nasD, nasB
Sequences Protein DNA DNA_with_flanks
Genetic context
NasC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NasC expression.png


























Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU03310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
  • Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

Original publications