nasC

168

nitrate reductase (catalytic subunit)

locus: BSU_03310

Molecular weight: 78.39 kDa

pI: 6.73

Protein length: 710 aa

Gene length: 2133 bp

function: utilization of nitrate

product: nitrate reductase (catalytic subunit)

essential: no

synonyms: nasC, narB, nasBB

List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

Gene

Coordinates: 358,303 → 360,435

Advertisement

The protein

Protein family

• [wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

[wiki|Domains]

• [wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 19-77) (according to UniProt)

[wiki|Cofactors]

• MoCo  [Pubmed|11289299]
• Fe-S cluster  [Pubmed|11289299]

Structure

• [PDB|2IV2] (from E. coli, 32% identity) [pubmed|16830149]
• [AF|P42434]

Biological materials

Mutant

• BKE03310 (Δ[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA,  downstream forward: _UP4_TAAATTTTTCATAAAATTTT
• BKK03310 (Δ[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA,  downstream forward: _UP4_TAAATTTTTCATAAAATTTT

References

Reviews

• 22103536,11289299

Original Publications

• 12823818,7868621,25755103,8799114,16885456,16830149