nasB

168

nitrate reductase (electron transfer subunit)

locus: BSU_03320

Molecular weight: 84.57 kDa

pI: 5.48

Protein length: 771 aa

Gene length: 2316 bp

function: utilization of nitrate

product: nitrate reductase (electron transfer subunit)

essential: no

synonyms: nasB, nasBA

List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)

Gene

Coordinates: 360,442  362,757

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The protein

[wiki|Cofactors]

• FAD  [Pubmed|11289299]

Structure

• [PDB|3KLJ] (from Clostridium acetobutylicum, corresponds to aa 2 ... 371, 27% identity) [pubmed|20017214]
• [AF|P42433]

Paralogous protein(s)

• [protein|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]

Biological materials

Mutant

• 1A971 ( ''nasB''::''erm''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A971&Search=1A971 BGSC]
• BKE03320 ([gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATCAGACCTCCTTTG,  downstream forward: _UP4_TGAGGCATTTTGACTGAACG
• BKK03320 ([gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATCAGACCTCCTTTG,  downstream forward: _UP4_TGAGGCATTTTGACTGAACG

References

Reviews

• 22103536,11289299

Original Publications

• 7836289,12823818,7868621,25755103,8799114,16885456,20017214