Difference between revisions of "NasA"

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* '''Description:''' nitrate transporter <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate uptake  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate uptake  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03330 nasA]
 
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
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|colspan="2" | '''Genetic context''' <br/> [[Image:nasA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nasA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasA_362937_364142_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:nasA_expression.png|500px]]
 
 
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* '''Operon:'''  
 
* '''Operon:'''  
  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasA_362937_364142_1 nasA] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''  
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* '''Regulation:'''  
 
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** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
 
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114 PubMed]
  
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* '''Additional information:'''
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* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =

Revision as of 12:05, 7 August 2012

  • Description: nitrate transporter

Gene name nasA
Synonyms
Essential no
Product nitrate transporter
Function nitrate uptake
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 45 kDa, 10.494
Gene length, protein length 1263 bp, 421 aa
Immediate neighbours nasB, yciA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NasA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

transporters/ other, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU03330

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s): NarK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

K Ogawa, E Akagawa, K Yamane, Z W Sun, M LaCelle, P Zuber, M M Nakano
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(5);1409-13
[PubMed:7868621] [WorldCat.org] [DOI] (P p)

M M Nakano, F Yang, P Hardin, P Zuber
Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(3);573-9
[PubMed:7836289] [WorldCat.org] [DOI] (P p)

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