Difference between revisions of "NasA"

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Revision as of 12:31, 16 May 2013

  • Description: nitrate transporter

Gene name nasA
Synonyms
Essential no
Product nitrate transporter
Function nitrate uptake
Gene expression levels in SubtiExpress: nasA
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 45 kDa, 10.494
Gene length, protein length 1263 bp, 421 aa
Immediate neighbours nasB, yciA
Sequences Protein DNA DNA_with_flanks
Genetic context
NasA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NasA expression.png
























Categories containing this gene/protein

transporters/ other, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU03330

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s): NarK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

Original publications

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

K Ogawa, E Akagawa, K Yamane, Z W Sun, M LaCelle, P Zuber, M M Nakano
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(5);1409-13
[PubMed:7868621] [WorldCat.org] [DOI] (P p)

M M Nakano, F Yang, P Hardin, P Zuber
Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(3);573-9
[PubMed:7836289] [WorldCat.org] [DOI] (P p)