Difference between revisions of "NadF"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 88 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 405 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:50, 17 April 2014

  • Description: NAD kinase

Gene name nadF
Synonyms yjbN, ppnK
Essential yes PubMed
Product inorganic polyphosphate/ATP-NAD kinase
Function generation of NADP from NAD
Gene expression levels in SubtiExpress: nadF
Metabolic function and regulation of this protein in SubtiPathways:
NadF
MW, pI 29 kDa, 5.991
Gene length, protein length 798 bp, 266 aa
Immediate neighbours sasB, yjbO
Sequences Protein DNA DNA_with_flanks
Genetic context
YjbN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NadF expression.png















Categories containing this gene/protein

biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
  • Protein family: NAD kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: allosterically activated by quinolinic acid

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: expressed during exponential growth
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 88 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 405 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544] [WorldCat.org] [DOI] (P p)

Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004] [WorldCat.org] [DOI] (P p)