Difference between revisions of "NadF"

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* '''Mutant:'''
 
* '''Mutant:'''
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** 1A825 ( ''nadF''::''erm''), {{PubMed|12682299}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A825&Search=1A825 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:29, 19 September 2012

  • Description: NAD kinase

Gene name nadF
Synonyms yjbN, ppnK
Essential yes PubMed
Product inorganic polyphosphate/ATP-NAD kinase
Function generation of NADP from NAD
Gene expression levels in SubtiExpress: nadF
MW, pI 29 kDa, 5.991
Gene length, protein length 798 bp, 266 aa
Immediate neighbours yjbM, yjbO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YjbN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NadF expression.png
























Categories containing this gene/protein

biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
  • Protein family: NAD kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: allosterically activated by quinolinic acid

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: expressed during exponential growth
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544] [WorldCat.org] [DOI] (P p)

Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004] [WorldCat.org] [DOI] (P p)