nadC

168

nicotinate-nucleotide diphosphorylase (carboxylating)

locus: BSU_27860

Molecular weight: 31.24 kDa

pI: 5.18

Protein length: 289 aa

Gene length: 870 bp

function: NAD biosynthesis

product: nicotinate-nucleotide diphosphorylase (carboxylating)

essential: no

ec: 2.4.2.19

synonyms: nadC, yrxB

List of homologs in different organisms, belongs to COG0157 (Galperin et al., 2021)

Gene

Coordinates: 2,847,048  2,847,917

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The protein

Catalyzed reaction/ biological activity

• CO₂ + diphosphate + nicotinate β-D-ribonucleotide --> 5-phospho-α-D-ribose 1-diphosphate + 2 H⁺ + quinolinate (according to UniProt)

Protein family

• nadC/modD family (single member, according to UniProt)

Structure

• [PDB|1X1O] (from Thermus thermophilus, 45% identity)
• [AF|P39666]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]

  description: [Pubmed|8444804]

  regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]

  regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]: repression, [Pubmed|16199587], in [regulon|protein:510DE075EA964D22C42DEB171509F6CDA600A402|nadR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8444804], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • An [wiki|search|ncRNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]

Biological materials

Mutant

• BKE27860 ([gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCTCAAGAAAAAAGTGATTC,  downstream forward: _UP4_TGTTATGTCAATTCTTGATG
• BKK27860 ([gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCTCAAGAAAAAAGTGATTC,  downstream forward: _UP4_TGTTATGTCAATTCTTGATG

References

• 8444804,16199587