murP

168

N-acetyl muramic acid permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIBC of the [category|SW.1.2.2|PTS]

locus: BSU_01680

Molecular weight: 47.44 kDa

pI: 8.5

Protein length: 455 aa

Gene length: 1368 bp

function: N-acetyl muramic acid uptake and phosphorylation

product: N-acetyl muramic acid [category|SW.1.2.2|PTS] permease, EIIBC component

essential: no

ec: 2.7.1.69

synonyms: murP, ybbF

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 189,790  191,157

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The protein

Catalyzed reaction/ biological activity

• transport and concomitant phosphorylation of N-acetyl muramic acid

Protein family

• [category|SW.1.2.2|PTS] permease, sucrose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIB domain] type-1 (aa 8-90) (according to UniProt)
• [wiki|PTS EIIC domain] type-1 (aa 116-455) (according to UniProt)

Structure

• [AF|Q797S1]

Paralogous protein(s)

• [protein|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP], [protein|531F132F7F6A878F1E1D56977B9898A14272349A|sacX], [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|sacP], [protein|BC568649A341B2E6341993EDA4BF52BDE18A3294|treP]

Biological materials

Mutant

• MGNA-B950 (ybbF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1949 NBRP B. subtilis, Japan]
• BKE01680 ([gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG
• BKK01680 ([gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG

References

• 36815589,10627040,15060041,22383849,30038046