Difference between revisions of "MurAA"

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(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
** pRSETA-murAA, Expression of His(6)-MurAA, avalable in [[Ulf Gerth]]'s lab {{PubMed|14763982}}  
 
** pRSETA-murAA, Expression of His(6)-MurAA, avalable in [[Ulf Gerth]]'s lab {{PubMed|14763982}}  
 +
**pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 09:26, 14 March 2014

  • Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Gene name murAA
Synonyms murA
Essential yes PubMed
Product UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murAA
Metabolic function and regulation of this protein in SubtiPathways:
murAA
MW, pI 46 kDa, 5.45
Gene length, protein length 1308 bp, 436 aa
Immediate neighbours spoIID, ywmB
Sequences Protein DNA DNA_with_flanks
Genetic context
MurAA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MurAA expression.png



















Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU36760

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
  • Protein family: MurA subfamily (according to Swiss-Prot)
  • Paralogous protein(s): MurAB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3SG1 (from B. anthracis, 79% identity, 94% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • pRSETA-murAA, Expression of His(6)-MurAA, avalable in Ulf Gerth's lab PubMed
    • pGP2597: (IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ankur Gautam, Praveen Rishi, Rupinder Tewari
UDP-N-acetylglucosamine enolpyruvyl transferase as a potential target for antibacterial chemotherapy: recent developments.
Appl Microbiol Biotechnol: 2011, 92(2);211-25
[PubMed:21822642] [WorldCat.org] [DOI] (I p)

Original publications