mtnW

168

2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein

locus: BSU_13590

Molecular weight: 44.89 kDa

pI: 5.28

Protein length: 414 aa

Gene length: 1245 bp

function: methionine salvage

product: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

essential: no

ec: null

synonyms: mtnW, ykrW

List of homologs in different organisms, belongs to COG1850 (Galperin et al., 2021)

Gene

Coordinates: 1,427,034  1,428,278

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The protein

Catalyzed reaction/ biological activity

• 5-methylsulfanyl-2,3-dioxopentyl phosphate --> 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate (according to UniProt)

Protein family

• RuBisCO large chain family (single member, according to UniProt)

Structure

• [PDB|2ZVI] [Pubmed|19690372]
• [AF|O31666]

Biological materials

Mutant

• BKE13590 ([gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT,  downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC
• BKK13590 ([gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT,  downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC

References

• 10094622,19279009,12022921,14551435,11914366,12107147,19194007,15102328,19690372,12787499,18039762