MtnU

• Description: omega-amidase
  
  

Gene name	mtnU
Synonyms	ykrU
Essential	no
Product	omega-amidase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnU
Metabolic function and regulation of this protein in SubtiPathways: mtnU
MW, pI	29 kDa, 5.099
Gene length, protein length	777 bp, 259 aa
Immediate neighbours	mtnK, mtnE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU13570

Phenotypes of a mutant

• reduced growth with 5-methylthioribose as single sulfur source PubMed

Database entries

• BsubCyc: BSU13570

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

the E.coli homolog: yafV

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 2-ketoglutaramate + H₂O ---> 2-oxoglutarate + NH₃ PubMed

• Protein family: CN hydrolase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13570

• Structure:

• UniProt: O31664

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnU PubMed

• Expression browser: mtnU PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 333 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 739 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: