MtnE

• Description: glutamine transaminase
  
  

Gene name	mtnE
Synonyms	ykrV
Essential	no
Product	glutamine transaminase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnE
Metabolic function and regulation of this protein in SubtiPathways: mtnE
MW, pI	43 kDa, 4.912
Gene length, protein length	1194 bp, 398 aa
Immediate neighbours	mtnU, mtnW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU13580

Phenotypes of a mutant

• reduced growth with 5-methylthioribose as single sulfur source PubMed

Database entries

• BsubCyc: BSU13580

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 2-ketomethylthiobutyrate + glutamine ---> 2-ketoglutaramate + methionine PubMed

• Protein family: MtnE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13580

• Structure:

• UniProt: O31665

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnE PubMed

• Expression browser: mtnE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 330 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 287 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kenneth W Ellens, Lynn G L Richardson, Océane Frelin, Joseph Collins, Cintia Leite Ribeiro, Yih-Feng Hsieh, Robert T Mullen, Andrew D Hanson
Evidence that glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants.
Phytochemistry: 2015, 113;160-9
[PubMed:24837359] [WorldCat.org] [DOI] (I p)

Agnieszka Sekowska, Valérie Dénervaud, Hiroki Ashida, Karine Michoud, Dieter Haas, Akiho Yokota, Antoine Danchin
Bacterial variations on the methionine salvage pathway.
BMC Microbiol: 2004, 4;9
[PubMed:15102328] [WorldCat.org] [DOI] (I e)

Bradley J Berger, Shane English, Gene Chan, Marvin H Knodel
Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.
J Bacteriol: 2003, 185(8);2418-31
[PubMed:12670965] [WorldCat.org] [DOI] (P p)

Agnieszka Sekowska, Antoine Danchin
The methionine salvage pathway in Bacillus subtilis.
BMC Microbiol: 2002, 2;8
[PubMed:12022921] [WorldCat.org] [DOI] (I e)