mtlD

168

mannitol-1-phosphate 5-dehydrogenase

locus: BSU_03990

Molecular weight: 40.31 kDa

pI: 6.29

Protein length: 373 aa

Gene length: 1122 bp

function: mannitol utilization

product: mannitol-1-phosphate 5-dehydrogenase

essential: no

ec: 1.1.1.17

synonyms: mtlD, mtlB

List of homologs in different organisms, belongs to COG0246 (Galperin et al., 2021)

Gene

Coordinates: 451,618  452,739

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The protein

Catalyzed reaction/ biological activity

• D-mannitol 1-phosphate + NAD⁺ --> β-D-fructose 6-phosphate + NADH (according to UniProt)

Protein family

• mannitol dehydrogenase family (with [protein|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB], according to UniProt)

Structure

• [PDB|3H2Z] (from ''Shigella flexneri 2a str. 2457t mutant'', 42% identity, 59% similarity)
• [AF|P42957]

Biological materials

Mutant

• MGNA-C019 (mtlD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2017 NBRP B. subtilis, Japan]
• BKE03990 ([gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA,  downstream forward: _UP4_TAACCGACCACCCGTGACAC
• BKK03990 ([gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA,  downstream forward: _UP4_TAACCGACCACCCGTGACAC

References

• 12897001,20444094,10627040,20525796