MscL

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  • Description: large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment

Gene name mscL
Synonyms ywpC
Essential no
Product large conductance mechanosensitive channel protein
Function resistance to osmotic downshockglycine betaine export
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 14 kDa, 4.649
Gene length, protein length 390 bp, 130 aa
Immediate neighbours ywpD, ywpB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MscL context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU36360

Phenotypes of a mutant

sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: mscL family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane PubMed

Database entries

  • Structure: 3HZQ (MscL of Staphylococcus aureus) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed in logarithmic phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Reviews

Valeria Vásquez, Eduardo Perozo
Structural biology: A channel with a twist.
Nature: 2009, 461(7260);47-9
[PubMed:19727188] [WorldCat.org] [DOI] (I p)

Christopher D Pivetti, Ming-Ren Yen, Samantha Miller, Wolfgang Busch, Yi-Hsiung Tseng, Ian R Booth, Milton H Saier
Two families of mechanosensitive channel proteins.
Microbiol Mol Biol Rev: 2003, 67(1);66-85, table of contents
[PubMed:12626684] [WorldCat.org] [DOI] (P p)

Original Publications

Thijs R H M Kouwen, Erik N Trip, Emma L Denham, Mark J J B Sibbald, Jean-Yves F Dubois, Jan Maarten van Dijl
The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.
Antimicrob Agents Chemother: 2009, 53(11);4702-11
[PubMed:19738010] [WorldCat.org] [DOI] (I p)

Zhenfeng Liu, Chris S Gandhi, Douglas C Rees
Structure of a tetrameric MscL in an expanded intermediate state.
Nature: 2009, 461(7260);120-4
[PubMed:19701184] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Ann E Cowan, Barbara Setlow, Peter Setlow
Levels and localization of mechanosensitive channel proteins in Bacillus subtilis.
Arch Microbiol: 2009, 191(5);403-14
[PubMed:19252899] [WorldCat.org] [DOI] (I p)

Thijs R H M Kouwen, Haike Antelmann, René van der Ploeg, Emma L Denham, Michael Hecker, Jan Maarten van Dijl
MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a hypotonic environment.
Proteomics: 2009, 9(4);1033-43
[PubMed:19160392] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, Erhard Bremer
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
Appl Environ Microbiol: 2008, 74(8);2454-60
[PubMed:18310427] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Peter Setlow
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.
Arch Microbiol: 2008, 189(1);49-58
[PubMed:17665170] [WorldCat.org] [DOI] (P p)

Paul G Wahome, Peter Setlow
The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis.
Arch Microbiol: 2006, 186(5);377-83
[PubMed:16897034] [WorldCat.org] [DOI] (P p)

P C Moe, P Blount, C Kung
Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.
Mol Microbiol: 1998, 28(3);583-92
[PubMed:9632260] [WorldCat.org] [DOI] (P p)