MreC

• Description: write here
  
  

Gene name	mreC
Synonyms
Essential	yes PubMed
Product	cell-shape determining protein
Function
MW, pI	32 kDa, 6.248
Gene length, protein length	870 bp, 290 aa
Immediate neighbours	mreD, mreB
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context

The gene

Basic information

• Coordinates: 2859062-2859931

Phenotypes of a mutant

mreC is essential under normal conditions PubMed. Depletion of MreC leads to a progressive increse in the width and a decreas in the length of the cell. This shape defect is consistent with a role for mreC in cell wall synthesis during elongation and similar to other genes with roles in elongation like rodA and the redundant gene pair pbpA and pbpH (also known as ykuA). Electron microscopy of cells depleted of MreC show regions of the cell where a thick and irregular cell wall has accumulated PubMed and PubMed. mreC can be deleted provided that 0.5 M sucrose and 20 mM Magnesium is provided in the media, mreC is therefore conditionally essentail. The phenotype of the mreC deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters PubMed.

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: None

• Domains: Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Interacts with MreD, and a subset of the PBPs PubMed

• Localization: GFP-MreC localises to the cell membrane in a helical pattern PubMed.

Database entries

• Structure:-
  • 2J5U: MreC from Lysteria monocytogenes PubMed
  • 2QF4: MreC monomer from Streptococcus pneumoniae PubMed
  • 2QF5: MreC dimer from Streptococcus pneumoniae PubMed

• Swiss prot entry: Q01466

• KEGG entry: K03570

• E.C. number:

Expression and regulation

• Operon: mreB-mreC-mreD-minC-minD

• Sigma factor: SigH PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the Errington lab PubMed.

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed