MprF

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  • Description: synthesis of lysylphosphatidylglycerol (L-PG), lysinylation of phosphatidylglycerol provides some resistance against positively charged antimicrobial peptides

Gene name mprF
Synonyms yfiW, yfiX
Essential no
Product lysyl-phosphatidylglycerol synthase
Function lysyl-phosphatidylglycerol lipid biosynthesis
Gene expression levels in SubtiExpress: mprF
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 96 kDa, 9.498
Gene length, protein length 2568 bp, 856 aa
Immediate neighbours yfiV, yfiY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YfiW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MprF expression.png




























Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU08425

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]

Additional information

Gene was re-assembled due to sequencing error in original sequence (it encompasses the genes annotated as yfiX and yfiW in SubtiList) PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: mprF (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hervé Roy, Michael Ibba
Broad range amino acid specificity of RNA-dependent lipid remodeling by multiple peptide resistance factors.
J Biol Chem: 2009, 284(43);29677-83
[PubMed:19734140] [WorldCat.org] [DOI] (I p)

Anna-Barbara Hachmann, Esther R Angert, John D Helmann
Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin.
Antimicrob Agents Chemother: 2009, 53(4);1598-609
[PubMed:19164152] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, John D Helmann
Phenotypic and transcriptomic characterization of Bacillus subtilis mutants with grossly altered membrane composition.
J Bacteriol: 2008, 190(23);7797-807
[PubMed:18820022] [WorldCat.org] [DOI] (I p)

Hervé Roy, Michael Ibba
RNA-dependent lipid remodeling by bacterial multiple peptide resistance factors.
Proc Natl Acad Sci U S A: 2008, 105(12);4667-72
[PubMed:18305156] [WorldCat.org] [DOI] (I p)

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

Petra Staubitz, Andreas Peschel
MprF-mediated lysinylation of phospholipids in Bacillus subtilis--protection against bacteriocins in terrestrial habitats?
Microbiology (Reading): 2002, 148(Pt 11);3331-3332
[PubMed:12427923] [WorldCat.org] [DOI] (P p)