MprF

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  • Description: synthesis of lysylphosphatidylglycerol (L-PG), lysinylation of phosphatidylglycerol provides some resistance against positively charged antimicrobial peptides

Gene name mprF
Synonyms yfiW, yfiX
Essential no
Product lysyl-phosphatidylglycerol synthase
Function lysyl-phosphatidylglycerol lipid biosynthesis
Gene expression levels in SubtiExpress: mprF
Metabolic function and regulation of this protein in SubtiPathways:
mprF
MW, pI 96 kDa, 9.498
Gene length, protein length 2568 bp, 856 aa
Immediate neighbours yfiV, yfiY
Sequences Protein DNA DNA_with_flanks
Genetic context
YfiW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MprF expression.png




























Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU08425

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]

Additional information

Gene was re-assembled due to sequencing error in original sequence (it encompasses the genes annotated as yfiX and yfiW in SubtiList) PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: mprF (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hervé Roy, Michael Ibba
Broad range amino acid specificity of RNA-dependent lipid remodeling by multiple peptide resistance factors.
J Biol Chem: 2009, 284(43);29677-83
[PubMed:19734140] [WorldCat.org] [DOI] (I p)

Anna-Barbara Hachmann, Esther R Angert, John D Helmann
Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin.
Antimicrob Agents Chemother: 2009, 53(4);1598-609
[PubMed:19164152] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, John D Helmann
Phenotypic and transcriptomic characterization of Bacillus subtilis mutants with grossly altered membrane composition.
J Bacteriol: 2008, 190(23);7797-807
[PubMed:18820022] [WorldCat.org] [DOI] (I p)

Hervé Roy, Michael Ibba
RNA-dependent lipid remodeling by bacterial multiple peptide resistance factors.
Proc Natl Acad Sci U S A: 2008, 105(12);4667-72
[PubMed:18305156] [WorldCat.org] [DOI] (I p)

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

Petra Staubitz, Andreas Peschel
MprF-mediated lysinylation of phospholipids in Bacillus subtilis--protection against bacteriocins in terrestrial habitats?
Microbiology (Reading): 2002, 148(Pt 11);3331-3332
[PubMed:12427923] [WorldCat.org] [DOI] (P p)