Difference between revisions of "MotA"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* loss of swimming and swarming motility {{PubMed|24296669}}
 +
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 
** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed|22821967}}
 
** the interaction with [[DgrA]] inhibits [[MotA]] activity {{PubMed|22821967}}
 +
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 
** [[MotA]]-[[MotB]]
 
** [[MotA]]-[[MotB]]
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=References=
 
=References=
<pubmed> 6313226 21821766 22821967</pubmed>
+
<pubmed> 6313226 21821766 22821967 24296669 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:09, 4 December 2013

  • Description: H+-coupled MotA-MotB flagellar stator

Gene name motA
Synonyms mot
Essential no
Product motility protein (flagellar motor rotation)
Function motility and chemotaxis
Gene expression levels in SubtiExpress: motA
Interactions involving this protein in SubtInteract: MotA
MW, pI 29 kDa, 5.01
Gene length, protein length 810 bp, 270 aa
Immediate neighbours motB, clpE
Sequences Protein DNA DNA_with_flanks
Genetic context
MotA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MotA expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU13690

Phenotypes of a mutant

  • loss of swimming and swarming motility PubMed
  • mucoid phenotype due to the DegU-P activated overexpression of the pgsB-pgsC-pgsA operon and resulting overproduction of poly-gamma-glutamate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: motA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226] [WorldCat.org] [DOI] (P p)