MmgE

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  • Description: putative methyl-cis-aconitase

Gene name mmgE
Synonyms yqiP, prpD
Essential no
Product unknown
Function mother cell metabolism
Gene expression levels in SubtiExpress: mmgE
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 52 kDa, 6.111
Gene length, protein length 1416 bp, 472 aa
Immediate neighbours yqiQ, mmgD
Sequences Protein DNA DNA_with_flanks
Genetic context
MmgE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MmgE expression.png




























Categories containing this gene/protein

sporulation proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

  • Locus tag: BSU24130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O (according to Swiss-Prot)
  • Protein family: prpD family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains an iron-sulfur cluster
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)