MmgA

• Description: degradative acetoacetyl-CoA thiolase
  
  

Gene name	mmgA
Synonyms	yqiL
Essential	no
Product	degradative acetoacetyl-CoA thiolase
Function	mother cell metabolism, leucine utilization
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	41 kDa, 5.904
Gene length, protein length	1179 bp, 393 aa
Immediate neighbours	mmgB, yqiK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins, utilization of amino acids

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU24170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA PubMed

• Protein family: thiolase family (according to Swiss-Prot)

• Paralogous protein(s): FadA, YhfS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3SS6 (the protein of B. anthracis, 44% identity, 73% similarity)

• UniProt: P45855

• KEGG entry: [3]

• E.C. number: 2.3.1.9

Expression and regulation

• Operon: mmgA-mmgB-mmgC-mmgD-mmgE-yqiQ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Jason J Reddick, Jayme K Williams
The mmgA gene from Bacillus subtilis encodes a degradative acetoacetyl-CoA thiolase.
Biotechnol Lett: 2008, 30(6);1045-50
[PubMed:18246303] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)